Abstract: Objective:Simulate a typical weather process and compare the levels of Apo E Knockout Mice' HSP60, SOD, TNF, sICAM-1, HIF-1α, ET-1, NO, body weight and rectal temperature to study the effects of hot and cold stimulation in mice.In order to further investigate the impact of heat wave on cardiovascular disease, eighteen Apo E Knockout Mice were divided into the heat wave group, strong cooling group and control group.The body weight and rectal temperature of each mouse were measured every day.By use of meteorological environment simulation box, the process of heat wave was stimulated, determining HSP60, SOD, TNF, sICAM-1, HIF-1α, ET-1, NO of Apo E Knockout Mice, measured by enzyme linked immunosorbent assay(ELISA).The result is as follows, HSP60, SOD, TNF, sICAM-1, HIF-1α and rectal temperature of Apo E Knockout Mice in group of heat wave were higher than that in strong cooling and control group.Body weight showed no significant difference but risk increased after the simulation process in strong cooling group.Strong cooling will cause vasoconstriction.Conclusion:Thermal stimulation may aggravate atherosclerosis disease development.The process of Strong Cooling may greatly increase the risk of coronary heart disease.